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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Related Experiment Video

Updated: May 18, 2026

Studying Cell Death Initiation Using a Digital Microscope
06:06

Studying Cell Death Initiation Using a Digital Microscope

Published on: November 10, 2023

Cell death detection by quantitative three-dimensional single-cell tomography.

Nai-Chia Cheng1, Tsung-Hsun Hsieh, Yu-Ta Wang

  • 1Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei, Taiwan.

Biomedical Optics Express
|October 2, 2012
PubMed
Summary
This summary is machine-generated.

Ultrahigh-resolution optical coherence tomography (UR-OCT) noninvasively images single cancer cells. This advanced technique accurately detects cell death in basal cell carcinoma (BCC) at the cellular level.

Keywords:
(100.2960) Image analysis(170.1530) Cell analysis(170.1870) Dermatology(170.4500) Optical coherence tomography

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Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
10:33

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

Published on: June 16, 2019

Area of Science:

  • Biomedical Optics
  • Cancer Research
  • Cell Biology

Background:

  • Basal cell carcinoma (BCC) is a common skin cancer.
  • Current methods for analyzing cancer cells can be invasive or lack cellular resolution.
  • Need for noninvasive, high-resolution techniques to study cancer cell dynamics.

Purpose of the Study:

  • To investigate the utility of ultrahigh-resolution optical coherence tomography (UR-OCT) for in vitro analysis of single-cell basal cell carcinoma (BCC).
  • To demonstrate the capability of UR-OCT in identifying and assessing cell death at the single-cell level.
  • To develop automated methods for quantitative analysis of cellular parameters using UR-OCT data.

Main Methods:

  • Application of ultrahigh-resolution optical coherence tomography (UR-OCT) for noninvasive, label-free, in situ imaging of BCC cells.
  • Three-dimensional (3D) analysis of cellular scattering properties with cellular spatial resolution.
  • Development of an automated method for extracting characteristic cellular parameters from 3D UR-OCT data volumes.
  • Quantitative comparison and parametric analysis of live and dead BCC cells.

Main Results:

  • UR-OCT successfully provided deep imaging depth and cellular spatial resolution for BCC cells in vitro.
  • Live and dead BCC cells were distinguishable based on morphological observations from 3D UR-OCT images.
  • The developed automated method enabled quantitative analysis of single-cell parameters.
  • UR-OCT demonstrated a clear capability to detect cell death at the individual cell level.

Conclusions:

  • UR-OCT is a powerful noninvasive tool for studying single cancer cells, including BCC.
  • The technique allows for label-free, 3D morphological analysis and differentiation of cell viability.
  • Automated parameter extraction enhances quantitative insights into cellular changes, particularly cell death detection.