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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
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Published on: March 20, 2015

Inner filter effect on surface enhanced Raman spectroscopic measurement.

Fathima S Ameer1, Siyam M Ansar, Wenfang Hu

  • 1Department of Chemistry, Mississippi State University, Mississippi State, Mississippi 39762, United States.

Analytical Chemistry
|October 3, 2012
PubMed
Summary

Gold nanoparticles (AuNPs) can enhance or reduce Raman signals depending on the analyte, impacting surface-enhanced Raman spectroscopy (SERS) measurements. AuNP aggregation significantly alters this inner filter effect and SERS enhancement.

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Area of Science:

  • Nanotechnology
  • Spectroscopy
  • Materials Science

Background:

  • Surface-enhanced Raman spectroscopy (SERS) is a powerful analytical technique.
  • Gold nanoparticles (AuNPs) are widely used to enhance SERS signals.
  • The inner filter effect of AuNPs on SERS is not fully understood.

Purpose of the Study:

  • To investigate the impact of the gold nanoparticle (AuNP) inner filter effect on surface-enhanced Raman spectroscopic (SERS) measurements.
  • To elucidate the relationship between AuNP aggregation and SERS signal variation.
  • To develop a reliable method for determining SERS enhancement factors.

Main Methods:

  • Utilized a bianalyte strategy with dithiopurine (DTP) and ethanol as model analytes.
  • Performed combined time-resolved UV-vis and Raman spectroscopy.
  • Employed experimental and computational approaches.

Main Results:

  • AuNPs enhanced the Raman signal of DTP while attenuating the signal of ethanol.
  • AuNP aggregation had a significant and opposing effect on the inner filter effect and SERS enhancement.
  • Demonstrated a clear correlation between AuNP aggregation state and SERS signal modulation.

Conclusions:

  • The inner filter effect of AuNPs is analyte-dependent and influenced by aggregation.
  • AuNP aggregation critically impacts SERS measurements, causing both enhancement and attenuation.
  • The study provides a simple methodology for accurate SERS enhancement factor determination.