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Related Concept Videos

Controlled-Current Coulometry: Overview01:27

Controlled-Current Coulometry: Overview

Controlled current coulometry, also known as amperostatic coulometry, is a technique used in electrochemical analysis to measure the quantity of a substance through the controlled passage of current. It involves the application of a constant current to an electrochemical cell containing the analyte of interest. As the current flows through the cell, the analyte undergoes a redox reaction at the electrode surface, resulting in a charge transfer. By monitoring the time required for a certain...
Controlled-Current Coulometry: Coulometric Titration01:18

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Coulometric titrations are a form of titrimetric analysis where the reagent is generated electrically, and its amount is evaluated based on current and generating time. The electron serves as the standard reagent. The procedure is similar to conventional titrations, such as endpoint detection.
The fundamental requirements for coulometric titrations are (1) 100% efficiency in the reagent-generating electrode reaction and (2) a stoichiometric and preferably rapid reaction between the generated...

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Preparation of Janus Particles and Alternating Current Electrokinetic Measurements with a Rapidly Fabricated Indium Tin Oxide Electrode Array
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Alternating current cloud point extraction on a microchip: a comprehensive study.

Naoki Sasaki1, Azusa Takemura, Kae Sato

  • 1Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Bunkyo, Tokyo, Japan. sasakina@fc.jwu.ac.jp

Electrophoresis
|October 3, 2012
PubMed
Summary
This summary is machine-generated.

This study optimizes alternating current cloud point extraction (ACPE) on a microchip for biomolecule preconcentration. Key findings include an amplitude threshold and optimal flow velocity and surfactant concentration for efficient extraction.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Microfluidics

Background:

  • Membrane-associated biomolecules require efficient preconcentration for analysis.
  • Microchip-based extraction techniques offer advantages in sample handling and analysis speed.

Purpose of the Study:

  • To comprehensively study and optimize alternating current cloud point extraction (ACPE) on a microchip.
  • To establish guidelines for ACPE application in microfluidic systems.

Main Methods:

  • ACPE experiments were conducted on a microchip.
  • Systematic variation of experimental parameters: voltage amplitude and frequency, flow velocity, surfactant, analyte, and salt concentrations.
  • Temperature control of microfluidic devices.

Main Results:

  • An amplitude threshold of 15 V(p-p) was identified for efficient extraction.
  • Optimal flow velocity (0.10–0.67 mm s⁻¹) and surfactant concentration (0.10–1.0%) were determined.
  • Extraction efficiency was independent of analyte concentration but sensitive to salt concentration (0.050–0.15 mol dm⁻³).
  • Frequency dependence was insignificant above 5 MHz.

Conclusions:

  • ACPE is an effective microchip technique for preconcentrating membrane-associated biomolecules.
  • Defined operational parameters provide a framework for optimizing ACPE in microfluidic analyses.
  • The study offers practical guidelines for utilizing ACPE in chemical and biochemical assays.