Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Impact of the β correction factor on the accuracy of speckle contrast imaging measurements.

Biomedical optics express·2026

Same author

Comparative Evaluation of R134a and HFO-1234ze Cryogen Spray Cooling Using a Mouse Model With Controllable Epidermal Pigmentation.

Lasers in surgery and medicine·2026

Same author

Two-step regression analysis approach to assess burn wound severity using spatial frequency domain imaging.

Journal of biomedical optics·2026

Same author

Effects of O<sup>+</sup> and a Non-O<sup>+</sup> Blood Type, Number Concentration, and Membrane Phosphatidylserine Flipping on the Circulation Dynamics and Biodistribution of Microsized Erythrocyte-Derived Optical Particles in Mice.

ACS applied bio materials·2025

Same author

CDC42-effector interaction inhibitors alter patterns of vessel arborization in skin and tumors <i>in vivo</i>.

iScience·2025

Same author

Improved temporal speckle contrast model for slow and fast dynamic: effect of temporal correlation among neighboring pixels.

Journal of biomedical optics·2025

Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026

Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026

Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026

Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026

Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026

Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 18, 2026

How to Build a Laser Speckle Contrast Imaging (LSCI) System to Monitor Blood Flow

How to Build a Laser Speckle Contrast Imaging (LSCI) System to Monitor Blood Flow

Published on: November 11, 2010

Laser speckle imaging using a consumer-grade color camera.

Owen Yang¹, Bernard Choi

¹Department of Biomedical Engineering, University of California, Irvine, 3120 Natural Sciences II, Irvine, California 92697, USA. yango@uci.edu

|October 3, 2012

Summary

This summary is machine-generated.

Digital single-lens reflex (dSLR) cameras can be used for laser speckle imaging (LSI), offering similar sensitivity to scientific cameras. Optimizing dSLR exposure settings is key for effective blood flow imaging.

More Related Videos

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Published on: August 5, 2009

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation

Published on: August 30, 2017

Related Experiment Videos

Last Updated: May 18, 2026

How to Build a Laser Speckle Contrast Imaging (LSCI) System to Monitor Blood Flow

How to Build a Laser Speckle Contrast Imaging (LSCI) System to Monitor Blood Flow

Published on: November 11, 2010

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Published on: August 5, 2009

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation

A Novel Approach to Overcome Movement Artifact When Using a Laser Speckle Contrast Imaging System for Alternating Speeds of Blood Microcirculation

Published on: August 30, 2017

Area of Science:

Optical imaging
Biomedical optics
Laser speckle imaging

Background:

Laser speckle imaging (LSI) is a noninvasive technique for mapping particle movement.
Traditionally, LSI uses scientific-grade monochrome cameras.
Digital single-lens reflex (dSLR) cameras offer potential alternatives.

Purpose of the Study:

To evaluate the feasibility of using a color dSLR camera for LSI.
To investigate the impact of dSLR exposure settings on speckle contrast.
To compare LSI performance between a dSLR and a scientific camera.

Main Methods:

Assessed speckle contrast variations with changes in dSLR aperture, exposure time, and ISO.
Conducted an in vivo reactive hyperemia experiment.
Compared blood flow dynamics visualized by a color dSLR and a monochrome scientific camera.

Main Results:

Demonstrated that a color dSLR camera can achieve comparable sensitivity to scientific cameras for LSI.
Showcased the influence of aperture, exposure time, and ISO on speckle contrast.
Confirmed qualitative similarity in blood flow visualization between dSLR and scientific cameras.

Conclusions:

Color dSLR cameras are a viable option for laser speckle imaging.
dSLR camera settings significantly affect speckle contrast and image quality.
dSLR cameras provide a cost-effective alternative for noninvasive blood flow monitoring.