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Updated: May 18, 2026

Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase
Published on: December 19, 2011
Back-scattering interferometry: an ultrasensitive method for the unperturbed detection of
Gabrielle L Haddad1, Sherri C Young, Ned D Heindel
1Department of Chemistry, Lehigh University, Bethlehem, PA 18015, USA.
Abstract:
A series of inhibitors of acetylcholinesterase (AChE) have been screened by back-scattering interferometry (BSI). Enzyme levels as low as 100 pM (22,000 molecules of AChE) can be detected. This method can be used to screen for mixed AChE inhibitors, agents that have shown high efficacy against Alzheimer's disease, by detecting dual-binding interactions. E = enzyme, I = inhibitor, S = substrate.
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