Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Quantifying protein unfolding kinetics with a high-throughput microfluidic platform.

bioRxiv : the preprint server for biology·2025

Same author

Viral co-infection, autoimmunity, and CSF HIV antibody profiles in HIV central nervous system escape.

Journal of neuroimmunology·2023

Same author

Integrating central nervous system metagenomics and host response for diagnosis of tuberculosis meningitis and its mimics.

Nature communications·2022

Same author

High throughput and quantitative enzymology in the genomic era.

Current opinion in structural biology·2021

Same author

Revealing enzyme functional architecture via high-throughput microfluidic enzyme kinetics.

Science (New York, N.Y.)·2021

Same author

Spontaneous emulsification induced by nanoparticle surfactants.

The Journal of chemical physics·2020

Search research articles

Related Experiment Video

Updated: May 18, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Programmable microfluidic synthesis of spectrally encoded microspheres.

R E Gerver¹, R Gómez-Sjöberg, B C Baxter

¹UC San Francisco/UC Berkeley Joint Graduate Group in Bioengineering, San Francisco, CA 94158-2517, USA.

|October 9, 2012

Summary

Researchers developed spectrally encoded fluorescent beads using lanthanide nanophosphors for biological assays. This novel platform enables highly multiplexed detection with minimal error, paving the way for advanced diagnostic tools.

More Related Videos

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

Published on: May 10, 2018

Fluorescence detection methods for microfluidic droplet platforms

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Related Experiment Videos

Last Updated: May 18, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals

Published on: May 10, 2018

Fluorescence detection methods for microfluidic droplet platforms

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Area of Science:

Biotechnology
Materials Science
Analytical Chemistry

Background:

Spectrally encoded fluorescent beads offer potential for miniaturized biological assays.
Multiplexing assays is crucial for high-throughput biological analysis.

Purpose of the Study:

To synthesize novel hydrophilic polymer beads encoded with lanthanide nanophosphors.
To develop a scalable platform for high-density spectral multiplexing in biological assays.

Main Methods:

Utilized a fully automated microfluidic device for bead synthesis.
Incorporated varying ratios of two lanthanide nanophosphors relative to a reference nanophosphor for spectral encoding.
Developed a method to generate 24 distinct spectral codes with high precision.

Main Results:

Synthesized hydrophilic PEG-acrylate polymer beads encoded with lanthanide nanophosphors.
Achieved distinct spectral codes with variations less than 3% from target values.
Demonstrated high distinguishability between codes with an error rate below 0.1%.

Conclusions:

The developed spectrally encoded beads are a viable platform for assay miniaturization and multiplexing.
The synthesis strategy is highly extensible, potentially enabling millions of unique codes.
This technology advances the field of high-throughput biological assays and diagnostics.