Related Experiment Video
Updated: May 17, 2026
11:25
Isolation and Culture of Mouse Cortical Astrocytes
Published on: January 19, 2013
Isolation of neonatal rat cortical astrocytes for primary cultures
J W Allen1, L A Mutkus, M Aschner
1Wake Forest University School of Medicine, Winston-Salem, North Carolina, USA.
Current Protocols in Toxicology
|October 10, 2012
Abstract:
There is increasing interest in the role of astrocytes as mediators of neurotoxicity. This unit describes a method for preparing astrocyte cultures of greater than 95% purity by enzymatic dissociation from neonatal rat brain. These preparations have both high yield and high viability.
