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Updated: May 17, 2026

A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
Published on: May 5, 2009
Analytical cytology: applications to neurotoxicology
M C Catlin1, M Guizzetti, R A Ponce
1University of Washington, Seattle, Washington, USA.
Abstract:
This unit describes methods for analyzing the effects of neurotoxicants on cell cycle regulation by dual parameter flow cytometry and on cell signaling by quantifying intracellular calcium concentrations within individual cells by scanning confocal laser microscopy or using the fluorescent calcium probe fluo-3.
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