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Updated: May 17, 2026

03:35
Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
Published on: June 28, 2024
HPLC-based assays for enzymes of glutathione biosynthesis
C C White1, C J Krejsa, D L Eaton
1University of Washington, Seattle, Washington, USA.
Current Protocols in Toxicology
|October 10, 2012
Abstract:
Glutamate cysteine ligase and glutathione synthase carry out the two-step synthesis of glutathione. The fluorescent thiol-reactive compound monobromobimane is used to derivatize reaction products in an HPLC-based assay with fluorescence detection. The assay described in this unit can be adapted for tissue homogenates or cultured cells.

