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Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
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Measurements of intracellular free calcium concentration in biological systems

K K Gunter1, T E Gunter

  • 1University of Rochester, Rochester, New York, USA.

Current Protocols in Toxicology
|October 10, 2012
PubMed

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Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...