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Updated: May 17, 2026
10:59
Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
Published on: August 21, 2021
Assays for DNA damage
1New York University School of Medicine, New York, New York, USA.
Current Protocols in Toxicology
|October 10, 2012
Abstract:
This unit describes several assays for detecting several kinds of DNA damage (strand breaks, internal crosslinking, DNA/protein crosslinks) and repair activity following exposure to genotoxic agents. The methods include single-cell electrophoresis (comet assay), filter eluting, K-SDS precipitation, and measurement of unscheduled DNA synthesis.
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