Related Experiment Video
Updated: May 17, 2026

Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays
Published on: March 7, 2025
Evaluation of the cytochrome b(5)/cytochrome b(5) reductase pathway
Lauren A Trepanier1, Sunil U Bajad, Joseph R Kurian
1University of Wisconsin-Madison, Madison, WI, USA.
Abstract:
NADH cytochrome b(5) reductase (b(5)R; EC 1.6.2.2; Diaphorase I; NADH: ferricytochrome b(5) oxidoreductase) is an FAD-containing protein, which, along with the hemoprotein cytochrome b(5) (cyt b(5)), mediates electron transfer from NADH to fatty acid desaturases, P450 oxidases, methemoglobin, and ascorbyl free radical. In addition, b(5)R and cyt b(5) can directly catalyze the reduction of hydroxylamine and amidoxime metabolites. This unit provides protocols for measuring the activity and mRNA expression of the cytochrome b(5)/cytochrome b(5) reductase pathway, and for obtaining heterologous expression and purification of the soluble forms of each protein.
Related Concept Videos
Electron Transport Chain: Complex III and IV
Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...
Electron Transport Chains
The ETC is comprised of...
Role of Reduced Coenzymes NADH and FADH₂
Redox Reactions
Electron Transport Chain Components

