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Related Experiment Video

Updated: May 17, 2026

Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes

Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes

Published on: July 28, 2017

RNA-binding protein AUF1 represses Dicer expression.

Kotb Abdelmohsen¹, Kumiko Tominaga-Yamanaka, Subramanya Srikantan

¹Laboratory of Molecular Biology and Immunology, National Institute on Aging-Intramural Research Program, NIH, 251 Bayview Blvd., Baltimore, MD 21224, USA. abdelmohsenk@mail.nih.gov

Nucleic Acids Research

|October 16, 2012

Summary

AUF1 (AU-binding factor 1) suppresses microRNA (miRNA) production by binding to DICER1 mRNA. This interaction reduces Dicer protein levels, thereby lowering mature miRNA synthesis.

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Last Updated: May 17, 2026

Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes

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Published on: July 28, 2017

Inducible and Reversible Dominant-negative (DN) Protein Inhibition

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Published on: January 7, 2019

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

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Published on: August 9, 2019

Area of Science:

Molecular Biology
Gene Regulation

Background:

MicroRNA (miRNA) biogenesis is crucial for cellular function and is tightly regulated.
Dicer is essential for processing precursor miRNAs into mature miRNAs.
Mechanisms regulating Dicer expression remain incompletely understood.

Purpose of the Study:

To investigate the role of RNA-binding protein AUF1 (AU-binding factor 1) in regulating Dicer expression.
To elucidate the interaction between AUF1 and DICER1 mRNA.

Main Methods:

Investigated the association of AUF1 with endogenous DICER1 mRNA.
Analyzed AUF1 interaction sites on DICER1 mRNA (coding region and 3'-UTR).
Assessed the impact of AUF1 modulation (silencing and overexpression) on DICER1 mRNA stability, Dicer levels, and miRNA synthesis.

Main Results:

AUF1 directly binds to DICER1 mRNA in both coding and 3'-UTR regions.
AUF1 binding decreases DICER1 mRNA stability and lowers Dicer protein levels.
Modulating AUF1 levels inversely affects Dicer expression and mature miRNA levels, without impacting pre-miRNA levels.

Conclusions:

AUF1 acts as a negative regulator of miRNA biogenesis.
AUF1 suppresses miRNA production by destabilizing DICER1 mRNA, leading to reduced Dicer levels.