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mRNA expression levels among cell regulatory and DNA damage genes in benzene-exposed workers in China
1Department of Occupational and Environmental Medicine, School of Public Health, Fudan University, Shanghai, China.
Objective:
We investigated how cells respond to the induction of DNA damage, focusing specifically on mRNA expression levels of cell regulatory and DNA repair genes under exposure to benzene.
Method:
The study sample was classified into three groups: direct exposure to benzene (A), indirect exposure to benzene (B), and non-exposed (C). Concentrations of benzene in the air of workplaces were monitored. Further blood biochemical parameters, cell cycle-regulated and DNA damage-related genes expression were analyzed.
Results:
The mRNA expression levels of Apel, Rad51, Bcl-2, Bax, Xpa, and Xpc genes were significantly down-regulated in groups A and B, with a dramatic up-regulation of p21 gene in group A accompanied by significantly lower counts of white blood cells, hemoglobin, platelets and lymphocyte subsets of CD8+, CD4+, T and B cells.
Conclusion:
The results indicated that exposure to benzene had significantly altered mRNA expression of some critical cell regulatory and DNA repair genes.
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