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Related Concept Videos

Hepatitis01:25

Hepatitis

Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver. The...
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Hepatic Portal System

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Related Experiment Video

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A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
10:28

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks

Published on: June 26, 2020

Culture systems for hepatitis E virus.

Hiroaki Okamoto1

  • 1Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan. hokamoto@jichi.ac.jp

Journal of Gastroenterology
|October 30, 2012
PubMed
Summary
This summary is machine-generated.

Developing efficient cell culture systems for hepatitis E virus (HEV) enables high-titer production of infectious HEV. This breakthrough facilitates research into HEV strains from humans and animals and reveals mechanisms of virus release.

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Area of Science:

  • Virology
  • Hepatitis E Virus (HEV) Research
  • Cell Culture Systems

Background:

  • Efficient cell culture systems for hepatitis E virus (HEV) were previously lacking, hindering detailed virus analysis.
  • HEV infects humans, pigs, and wild boar, posing a significant public health concern.
  • Understanding HEV replication and egress is crucial for developing effective treatments and control strategies.

Purpose of the Study:

  • To review the development of efficient cell culture systems for various HEV strains.
  • To detail a new model for HEV virion egress.
  • To extend the utility of cell culture systems to diverse HEV isolates across species.

Main Methods:

  • Established efficient HEV cell culture systems using human hepatocellular carcinoma (PLC/PRF/5) and lung cancer (A549) cell lines.
  • Inoculated cell cultures with HEV strains from human, pig, and wild boar samples (fecal, serum, liver).
  • Engineered infectious HEV cDNA clones of wild-type and variant strains.
  • Analyzed HEV particle association with lipids and ORF3 protein.
  • Investigated the interaction of HEV ORF3 protein with tumor susceptibility gene 101 (TSG101) and its role in multivesicular body (MVB) pathway involvement.

Main Results:

  • Developed high-titer infectious HEV production in cell culture systems.
  • Successfully cultured HEV strains from diverse human, pig, and wild boar sources, demonstrating broad applicability.
  • Identified association of cell culture-generated HEV particles with lipids and ORF3 protein, suggesting roles in assembly and release.
  • Demonstrated HEV ORF3 protein interaction with TSG101 via the PSAP motif, implicating the MVB pathway in HEV egress.

Conclusions:

  • Efficient HEV cell culture systems are now available for a wide range of strains from different hosts.
  • The study provides a novel model for HEV virion assembly and egress, involving host cell factors like TSG101 and the MVB pathway.
  • These advancements significantly facilitate HEV research, diagnostics, and therapeutic development.