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Interferences in clinical chemistry analysis
K S Saibaba1, M V Bhaskar, P V Rao
1Department of Biochemistry, Sri Venkateswara Institute of Medical Sciences (SVIMS), 517507 Tirupati.
Indian Journal of Clinical Biochemistry : IJCB
|October 30, 2012
Summary
Clinical chemistry analyses face interferences from various sources, impacting accuracy. This study highlights matrix interference between serum and aqueous calibrators, urging awareness and correction for quality assurance.
Area of Science:
- Clinical Chemistry
- Analytical Chemistry
- Laboratory Medicine
Background:
- Clinical chemistry laboratory analyses are susceptible to interferences affecting accuracy.
- Interferents originate from exogenous (drugs, additives) and endogenous (lipemia, hemolysis, icteria) sources.
Purpose of the Study:
- To investigate matrix interference in clinical chemistry.
- To demonstrate analytical bias between serum and aqueous matrix calibrators.
Main Methods:
- Comparative analysis of body fluid samples.
- Evaluation of analytical bias using different calibrator matrices.
Main Results:
- Significant matrix interference observed between serum and aqueous calibrators.
- Analytical bias identified as a key interferent impacting results.
Conclusions:
- Clinical chemists must be aware of matrix interference.
- Correction of interferences is crucial for quality assurance in laboratory testing.
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