Related Experiment Video
Updated: May 17, 2026
11:17
Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
Published on: August 30, 2018
Interferences in clinical chemistry analysis.
K S Saibaba1, M V Bhaskar, P V Rao
1Department of Biochemistry, Sri Venkateswara Institute of Medical Sciences (SVIMS), 517507 Tirupati.
Indian Journal of Clinical Biochemistry : IJCB
|October 30, 2012
Summary
Clinical chemistry analyses face interferences from various sources, impacting accuracy. This study highlights matrix interference between serum and aqueous calibrators, urging awareness and correction for quality assurance.
Area of Science:
- Clinical Chemistry
- Analytical Chemistry
- Laboratory Medicine
Background:
- Clinical chemistry laboratory analyses are susceptible to interferences affecting accuracy.
- Interferents originate from exogenous (drugs, additives) and endogenous (lipemia, hemolysis, icteria) sources.
Purpose of the Study:
- To investigate matrix interference in clinical chemistry.
- To demonstrate analytical bias between serum and aqueous matrix calibrators.
Main Methods:
- Comparative analysis of body fluid samples.
- Evaluation of analytical bias using different calibrator matrices.
Main Results:
- Significant matrix interference observed between serum and aqueous calibrators.
- Analytical bias identified as a key interferent impacting results.
Conclusions:
- Clinical chemists must be aware of matrix interference.
- Correction of interferences is crucial for quality assurance in laboratory testing.
Related Concept Videos
01:20Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and refractory oxide ion...
01:25Atomic Absorption Spectroscopy: Interference
Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
01:30Atomic Emission Spectroscopy: Interference
In atomic emission spectroscopy (AES), high-temperature atomizers excite a broad range of elements and molecules that generate complex emissions from sources such as oxides, hydroxides, and flame combustion products in the flame or plasma. Several strategies can be employed to minimize spectral interferences caused by overlapping emission lines or bands. These include increasing instrument resolution, choosing alternative emission lines, optimally placing the detector in low-background regions,...
01:24Automated Microbial Diagnostics
Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
00:56Blank Solutions
A blank solution is a solution that does not contain the analyte, or the substance of interest being tested or measured. It is typically prepared using the same reagents and procedure as the sample solution but without adding the analyte. The primary purpose of preparing a blank solution is to account for any background interference or contamination that may affect the accuracy and reliability of the analytical method.
In some experimental cases, the reagents, solvents, or lab equipment used in...
In some experimental cases, the reagents, solvents, or lab equipment used in...
01:25Pharmacokinetics: Drug–Drug Interactions
Drug interactions occur when the pharmacological effect of one drug is altered by another substance, either enhancing or diminishing its activity. The drug whose activity is altered is known as the object drug, and the substance causing the alteration is called the agent drug or the precipitant. The net effects of these interactions are mostly undesirable, leading to decreased effectiveness or increased adverse effects. In rare cases, interactions can be beneficial, such as the enhanced...