Effect of (-)epicatechin in modulating calicum-atpase activity in normal and diabetic human erythrocytes

Aqueous extract of the wood ofPterocarpus marsupium, commonly known as 'bijasar', is used in Indian Ayurveda system of medicine for treatment of diabetes. The active anti-diabetic principle in its aqueous extract has been found to be (-)epicatechin, a flavonoid. The present work was undertaken to study the effect of (-)epicatechin on erythrocyte membrane Ca(++)-ATPase from type 2 diabetic patients. The activity of erythrocyte Ca(++)-ATPase was significantly lower in type 2 diabetics.In vitro insulin treatment of erythrocyte ghosts, resulted in the increase of Ca(++)-ATPase activity in diabetic patients. Treatment with (-)epicatechin (1mM) resulted in an increase in the activity of erythrocyte Ca(++)-ATPase in both normal individuals and type 2 diabetic patients. The insulin like effect of (-)epicatechin on erythrocyte membrane Ca(++)-ATPase in type 2 diabetics is an interesting finding. Further work is needed to elucidate the mechanism of action of (-)epicatechin on modulation of erythrocyte membrane bound enzymes.