Modification of thyroid stimulating hormone estimation method in alpha prime LS system (SFRI)

The alpha prime LS (APLS) system consists of both enzyme linked immunosorbant assay (ELISA) and chemiluminescence immunoassay (CLIA) processing arrangements. All the programmes are open and accessible to modification. But, as CLIA kits are system dedicated kits, so the option for user defined programme may only be utilized for modification as and when it is absolutely necessary. The author had, some problem during routine performances with thyroid stimulating hormone (TSH) Monobind, U.S.A, kits. So, the author modified the incubation temperature, time of decontamination, use of signal reagents and finally the calibration pattern.