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Fluorescent methods in the study of UV-induced changes in structural and functional state of human blood lymphocytes
V G Artyukhov1, O V Putintseva, V A Vdovina
1Voronezh State University, Voronezh, Russia. avg@main.vsu.ru
Abstract:
Structural and functional state of human blood lymphocytes after exposure to UV light (240-390 nm) in doses of 151-1359 J/m(2) was studied by methods of laser flow cytofluorometry, indirect immunofluorescence, and fluorescent probes. Using a combination of these methods, we have showed that UV light in the specified doses induced changes in the surface phenotype of T cells: stimulation or suppression of the expression of antigen-recognizing receptor complex molecules (CD3, CD4, and CD8 markers) and their redistribution on the surface of immunocompetent cells (capping effect) with the formation of receptor clusters of various types.

