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Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry
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Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Published on: May 21, 2018

Post-translational modification in the archaea: structural characterization of multi-enzyme complex lipoylation

Mareike G Posner1, Abhishek Upadhyay, Susan J Crennell

  • 1Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, UK.

The Biochemical Journal
|November 3, 2012
PubMed
Summary

Archaeal lipoylation involves two proteins, LplA-N and LplA-C, which fold and interact to enable lipoic acid attachment. This study reveals their functional interdependence and substrate flexibility, crucial for understanding archaeal metabolism.

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