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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
¹H NMR of Labile Protons: Temporal Resolution01:10

¹H NMR of Labile Protons: Temporal Resolution

Protons bonded to heteroatoms such as nitrogen and oxygen exhibit a range of chemical shift values. This is due to the varying degree of hydrogen bonding between the proton and the heteroatom in other molecules. The extent of hydrogen bonding affects the electron density around the proton, thereby giving different chemical shift values for the protons in the proton NMR spectrum.
The –OH proton in alcohols typically appears in the range of δ 2 to 5 ppm but can vary depending on the specific...
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...

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Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

Time-resolved multiple probe spectroscopy.

G M Greetham1, D Sole, I P Clark

  • 1Central Laser Facility, Science and Technology Facilities Council, Research Complex at Harwell, Rutherford Appleton Laboratory, Harwell, Oxfordshire, OX11 0QX, United Kingdom. greg.greetham@stfc.ac.uk

The Review of Scientific Instruments
|November 7, 2012
PubMed
Summary
This summary is machine-generated.

Time-resolved multiple probe spectroscopy offers a powerful method for studying dynamic chemical processes. This technique captures picosecond to millisecond spectra in a single experiment, revealing detailed reaction kinetics.

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Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
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Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
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Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

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Area of Science:

  • Physical Chemistry
  • Spectroscopy
  • Chemical Dynamics

Background:

  • Studying rapid chemical reactions requires advanced spectroscopic techniques.
  • Existing methods may lack the time resolution or comprehensive data acquisition needed for complex dynamics.

Purpose of the Study:

  • To introduce and demonstrate a novel time-resolved multiple probe spectroscopy system.
  • To showcase its capability in analyzing dynamic processes from picosecond to millisecond timescales.

Main Methods:

  • Utilizing a single activation pulse with combined optical, electronic, and data acquisition.
  • Employing a pump-probe-probe-probe acquisition mode at 1 kHz pump and 10 kHz probe frequencies.
  • Monitoring the photolysis of tungsten hexacarbonyl (W(CO)6) via IR absorption spectroscopy.

Main Results:

  • Successfully measured picosecond vibrational cooling and microsecond bimolecular diffusion reactions.
  • Acquired comprehensive spectral data across multiple timescales within one experiment.
  • Demonstrated the system's versatility for studying diverse dynamic processes.

Conclusions:

  • Time-resolved multiple probe spectroscopy is a versatile tool for chemical dynamics.
  • The developed instrument provides high temporal resolution and rich data.
  • This technique facilitates in-depth understanding of reaction mechanisms and kinetics.