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Updated: May 17, 2026
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Freezing, Thawing, and Packaging Cells for Transport
Published on: July 2, 2008
Cryopreservation and thawing of cells
Wayne M Yokoyama1, Maria L Thompson2, Rolf O Ehrhardt2
1University of California School of Medicine, San Francisco, California.
Current Protocols in Immunology
|November 7, 2012
Summary
This study presents a simple, reproducible cell cryopreservation protocol that enhances cell viability and recovery. It avoids hazardous isopropanol, offering a cost-effective and safer alternative for cell lines, primary cells, and stem cell cultures.
Area of Science:
- Cell biology
- Cryobiology
- Biotechnology
Background:
- Cryopreservation is crucial for maintaining cell viability.
- Controlled freezing rates (e.g., 1°C/min) are essential for optimal cell recovery.
- Existing methods vary in cost, standardization, and safety, including electronic freezers, isopropyl alcohol containers, and rudimentary devices.
Purpose of the Study:
- To describe a simple, reproducible, and successful cryopreservation protocol.
- To eliminate the need for isopropanol in cell freezing.
- To provide a uniform method that improves cell viability and recovery.
Main Methods:
- A novel cryopreservation protocol is detailed.
- The method focuses on proper cell handling, sterility, and cryoprotective agent selection.
- It avoids the use of isopropanol.
Main Results:
- The described protocol is simple, reproducible, and successful for cell lines, primary cells, and stem cell cultures.
- It eliminates the costs and hazards associated with isopropanol use and disposal.
- The method provides uniform results with improved cell viability and recovery.
Conclusions:
- The developed protocol offers a safe, cost-effective, and efficient alternative for cell cryopreservation.
- This method enhances cell viability and recovery compared to traditional techniques.
- It is suitable for a range of cell types, including sensitive cultures.
