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Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
07:43

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

Site-specific gene integration in rice.

Vibha Srivastava1

  • 1Department of Crop, Soil & Environmental Sciences and Department of Horticulture, University of Arkansas, Fayetteville, AR, USA. vibhas@uark.edu

Methods in Molecular Biology (Clifton, N.J.)
|November 9, 2012
PubMed
Summary
This summary is machine-generated.

Site-specific gene integration ensures stable transgene expression in plants. Using systems like Cre-lox allows precise single-copy gene insertion, improving transgenic plant production.

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Area of Science:

  • Plant biotechnology
  • Molecular biology
  • Genetics

Background:

  • Conventional plant transformation often results in variable transgene expression due to multiple gene copies.
  • Unpredictable integration patterns lead to instability in transgene expression across generations.

Purpose of the Study:

  • To highlight the benefits of site-specific gene integration for stable transgene expression.
  • To present site-specific recombination systems as a method for precise gene insertion in plants.

Main Methods:

  • Utilizing site-specific recombination systems, such as the P1 bacteriophage Cre-lox system.
  • Engineering specific genomic sites for targeted foreign gene integration.

Main Results:

  • Site-specific integration leads to single-copy transgene insertions.
  • Transgenic plants generated through this method exhibit stable transgene expression over successive generations.

Conclusions:

  • Site-specific gene integration is a superior method for achieving stable transgene expression in plants.
  • This technique streamlines the production of reliable transgenic plants.