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Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium
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P2Y receptors and kidney function.

Volker Vallon1, James Stockand, Timo Rieg

  • 1Department of Medicine, University of California San Diego, San Diego, CA 92161, USA; VA San Diego Healthcare System, San Diego California, San Diego, CA 92161, USA; Department of Pharmacology, University of California San Diego, La Jolla, CA 92093, USA.

Wiley Interdisciplinary Reviews. Membrane Transport and Signaling
|November 13, 2012
PubMed
Summary
This summary is machine-generated.

The renal ATP/UTP/P2Y(2) receptor system regulates kidney cell volume and sodium-chloride (NaCl) and water homeostasis. This system inhibits arginine-vasopressin (AVP)-induced water reabsorption and epithelial sodium channel (ENaC) activity, impacting blood pressure.

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Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli
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Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli

Published on: June 27, 2015

Area of Science:

  • Nephrology
  • Cellular Physiology
  • Molecular Biology

Background:

  • Cellular release of nucleotides is crucial for cell function and integrity.
  • In the kidney, nucleotides act on P2Y receptors in response to cell volume or flow rate changes.
  • P2Y(2) receptors, activated by ATP and UTP, play a significant role in renal physiology.

Purpose of the Study:

  • To elucidate the role of the renal ATP/UTP/P2Y(2) receptor system in regulating kidney function.
  • To investigate the involvement of P2Y(2) receptors in cell volume, NaCl, and water homeostasis.
  • To understand the impact of this system on arginine-vasopressin (AVP) and epithelial sodium channel (ENaC) activity.

Main Methods:

  • Utilized gene knockout mice to study the function of P2Y(2) receptors.
  • Investigated the effects of AVP and cell volume changes on nucleotide release.
  • Examined the impact of increased NaCl intake on nucleotide release and P2Y(2) receptor activation.

Main Results:

  • P2Y(2) receptor activation by ATP/UTP inhibits AVP-induced cAMP formation and water reabsorption, stabilizing cell volume.
  • Increased NaCl intake leads to ATP/UTP release, activating apical P2Y(2) receptors to lower ENaC open probability and facilitate sodium excretion.
  • The ATP/UTP/P2Y(2) system inhibits ENaC activity during aldosterone escape and reduces NKCC2 expression/activity, while mediating vasodilation.

Conclusions:

  • The renal ATP/UTP/P2Y(2) receptor system is vital for preserving cell volume and integrity.
  • This system is intricately linked to body NaCl and water homeostasis, as well as blood pressure regulation.
  • P2Y(2) receptor signaling influences key transport proteins like ENaC and NKCC2 in the kidney.