Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preparation of gold probes.

Methods in molecular biology (Clifton, N.J.)·2012
Same author

Immunogold probes in electron microscopy.

Methods in molecular biology (Clifton, N.J.)·2012
Same author

Preparation of gold probes.

Methods in molecular biology (Clifton, N.J.)·2012
Same author

Immunogold probes for light microscopy.

Methods in molecular biology (Clifton, N.J.)·2012
Same author

Immunogold probes in electron microscopy.

Methods in molecular biology (Clifton, N.J.)·2012
Same author

Tumor necrosis factor (TNF)-alpha and interleukin (IL)-1beta down-regulate intercellular adhesion molecule (ICAM)-2 expression on the endothelium.

Cell adhesion and communication·1999

Related Experiment Video

Updated: May 16, 2026

Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses
09:49

Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses

Published on: July 14, 2014

Immunogold probes for light microscopy.

J E Beesley1

  • 1Wellcome Research Laboratories, Beckenham, UK.

Methods in Molecular Biology (Clifton, N.J.)
|November 15, 2012
PubMed
Summary
This summary is machine-generated.

Immunocytochemistry techniques use microscopic markers to locate antigens. Advances include immunofluorescence, immunoenzyme methods like peroxidase and alkaline phosphatase, and newer colloidal gold probes for light microscopy.

More Related Videos

High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses
09:24

High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses

Published on: February 18, 2016

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

Related Experiment Videos

Last Updated: May 16, 2026

Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses
09:49

Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses

Published on: July 14, 2014

High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses
09:24

High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses

Published on: February 18, 2016

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

Area of Science:

  • Immunocytochemistry
  • Microscopy
  • Biochemical Assays

Background:

  • Immunocytochemistry relies on antigen localization using microscopically detectable markers.
  • The field originated with immunofluorescence techniques for antigen detection.

Purpose of the Study:

  • To review the evolution and application of various immunocytochemical techniques.
  • To highlight the development and utility of different microscopic markers in antigen localization.

Main Methods:

  • Review of historical and contemporary immunocytochemical methodologies.
  • Description of immunofluorescence, immunoenzyme (peroxidase, alkaline phosphatase), and colloidal gold probe techniques.

Main Results:

  • Immunofluorescence and immunoenzyme techniques (peroxidase-antiperoxidase, alkaline phosphatase) are standard for immunohistochemistry.
  • Colloidal gold probes, initially for electron microscopy, are now applicable to light microscopy.

Conclusions:

  • Diverse microscopic markers have been developed to enhance antigen localization sensitivity and specificity.
  • Colloidal gold probes represent a recent advancement with potential in light microscope immunocytochemistry.