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Typing methods for legionella.

Christian Lück1, Norman K Fry, Jürgen H Helbig

  • 1Institute for Medical Microbiology and Hygiene, University of Technology, Dresden, Germany. Christian.Lueck@tu-dresden.de

Methods in Molecular Biology (Clifton, N.J.)
|November 15, 2012
PubMed
Summary
This summary is machine-generated.

This chapter details methods for subgrouping Legionella bacteria, including monoclonal antibody (mAb) techniques and genotyping methods like pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphism (AFLP), and sequence-based typing (SBT). These techniques aid in differentiating Legionella strains.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Immunology

Background:

  • Legionella species are significant human pathogens, necessitating accurate methods for strain differentiation.
  • Subgrouping Legionella is crucial for epidemiological investigations and understanding transmission dynamics.

Purpose of the Study:

  • To provide a comprehensive overview of current methods for subgrouping Legionella pneumophila and other Legionella species.
  • To describe both serological and molecular typing techniques for Legionella.

Main Methods:

  • Monoclonal antibody (mAb) subgrouping using indirect immunofluorescence and indirect ELISA.
  • Genotyping methods including pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphism (AFLP), and sequence-based typing (SBT).
  • Spoligotyping for distinguishing strains within the same sequence type (ST).

Main Results:

  • Monoclonal antibodies are available for noncommercial use from the authors.
  • Sequence-based typing (SBT) schema is established for L. pneumophila.
  • PFGE and AFLP are applicable to all Legionella species, offering broad utility.

Conclusions:

  • A range of standardized and reproducible methods exist for Legionella subgrouping and genotyping.
  • The choice of method depends on the specific application and Legionella species.
  • These techniques are essential tools for Legionella research and public health surveillance.