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Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
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Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
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Related Experiment Video

Updated: May 16, 2026

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence
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Detecting p53 isoforms at protein level.

Virginie Marcel1, Marie P Khoury, Kenneth Fernandes

  • 1CR-UK Cell Transformation Research Group, Centre for Oncology and Molecular Medicine, Ninewells Hospital, University of Dundee, Dundee, UK.

Methods in Molecular Biology (Clifton, N.J.)
|November 15, 2012
PubMed
Summary
This summary is machine-generated.

Detecting human p53 protein isoforms is crucial for understanding their role in gene expression and tumor suppression. This study provides methods for protein-level detection and functional analysis of p53 isoforms.

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Purification of Ubiquitinated p53 Proteins from Mammalian Cells
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Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Protein Isoform Analysis

Background:

  • Human p53 protein isoforms modulate tumor suppressor activity primarily through gene expression.
  • Understanding p53 isoform expression patterns is essential for studying the p53 network.
  • Current detection methods rely on antibody panels and molecular weight comparisons.

Purpose of the Study:

  • To describe protocols for detecting p53 protein isoforms at the protein level.
  • To provide a method for assessing the impact of p53 isoforms on transcriptional activity.

Main Methods:

  • Western blotting for p53 protein isoform detection.
  • Immunoprecipitation techniques.
  • Luciferase reporter gene assays for functional analysis.

Main Results:

  • Detailed protocols for Western blotting and immunoprecipitation of p53 isoforms are presented.
  • A luciferase assay method is described to evaluate p53 isoform-mediated transcriptional activity.

Conclusions:

  • The described methods facilitate the detection and functional study of p53 protein isoforms.
  • This work supports future research into the p53 network and its role in cancer.