Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
In vitro Mutagenesis01:16

In vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Materialogenetics: an emerging and promising framework for cell-specific genetic manipulation.

Bioactive materials·2026
Same author

Engineering Nanomaterials for the Treatment of Intervertebral Disc Degeneration: From Application to Therapeutic Mechanisms.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Constitutive activation of activin receptor-like kinase 3 in chondrocytes exacerbates skeletal dysplasia in mice with achondroplasia.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·2025
Same author

Genetic diversity and distribution patterns of Phaeocystis globosa in the eastern Chinese coastal seas: Significance of a global intraspecific re-examination.

Marine environmental research·2025
Same author

Characterization of a chemically induced osteoarthritis model in zebrafish.

Scientific reports·2025
Same author

Sensory-directed flavor analysis of Jinggu white tea: Exploring the formation mechanisms of sweet and fruity aromas.

Food chemistry: X·2024

Related Experiment Video

Updated: May 16, 2026

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
07:04

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Published on: February 5, 2019

Efficient multi-site-directed mutagenesis directly from genomic template.

Fengtao Luo1, Xiaolan Du, Tujun Weng

  • 1State Key Laboratory of Trauma, Burns and Combined Injury, Center of Bone Metabolism and Repair, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042 China.

Journal of Biosciences
|November 16, 2012
PubMed
Summary
This summary is machine-generated.

Researchers improved a DNA editing technique for complex genetic material like genomic DNA. This enhanced method allows for precise multi-site-directed mutagenesis, including fragment combinations, deletions, and insertions.

More Related Videos

Homemade Site Directed Mutagenesis of Whole Plasmids
07:11

Homemade Site Directed Mutagenesis of Whole Plasmids

Published on: May 11, 2009

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

Related Experiment Videos

Last Updated: May 16, 2026

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
07:04

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Published on: February 5, 2019

Homemade Site Directed Mutagenesis of Whole Plasmids
07:11

Homemade Site Directed Mutagenesis of Whole Plasmids

Published on: May 11, 2009

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Traditional multi-site-directed mutagenesis methods can be challenging with complex DNA templates.
  • Genomic DNA and complementary DNA present unique difficulties for precise genetic modification.

Purpose of the Study:

  • To enhance the overlap extension PCR method for improved multi-site-directed mutagenesis.
  • To develop a robust technique applicable to complex DNA templates like genomic DNA.

Main Methods:

  • Improvement of the overlap extension PCR technique.
  • Application of the enhanced method for mutagenesis on mouse genomic DNA.
  • Demonstration of DNA fragment combination, deletion, and insertion capabilities.

Main Results:

  • Successfully applied an improved multi-site-directed mutagenesis method to mouse genomic DNA.
  • The enhanced technique efficiently handles complex DNA templates.
  • Enabled precise DNA fragment manipulation, including insertions and deletions.

Conclusions:

  • The improved method offers a powerful tool for genetic engineering with complex DNA.
  • This technique facilitates efficient and accurate multi-site-directed mutagenesis in genomic DNA.
  • The method is versatile for various DNA modification strategies.