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Updated: May 16, 2026

Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System
Published on: August 17, 2017
Bacteriophages as templates for manufacturing supramolecular structures
Nuriye Korkmaz1, Young Jun Kim, Chang Hoon Nam
1Nanomedicine, Korea Institute of Science and Technology, Europe Forschungsgesellschaft mbH, Campus E 71, 66123 Saarbruecken, Germany. n.korkmaz@kist-europe.de
Abstract:
SS phages are genetically enginnered by replacing 2 N-terminal amino acids of the p8 coat protein of the fd phage. AGE and zeta potential measurements show that SS phages carry at least 1/4 less net negative surface charge than fd phages. Morphology and thickness of phages are studied at different counterion concentrations (10(-3) , 10(-2) and 10(-1) M) by AFM, SEM and immunofluorescence analyses. Bundles induced by CoCl2 and CaCl2 are either metallized by chemical reduction or biomineralized for apatite-like material formation. EDX spectroscopy confirms the presence of Co, P and Ca peaks in mineralized samples. Such bottom-up manufactured phage scaffolds might be applied in bioengineering studies.
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