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Updated: May 16, 2026
08:56
Visual Detection of Multiple Nucleic Acids in a Capillary Array
Published on: November 15, 2017
Detection of multiple waterborne pathogens using microsequencing arrays.
N E Brinkman1, R Francisco, T L Nichols
1National Exposure Research Laboratory, US Environmental Protection Agency, Cincinnati, OH, USA.
Journal of Applied Microbiology
|November 22, 2012
Summary
A new DNA microarray can detect multiple waterborne pathogens and indicator bacteria, including Cryptosporidium parvum and Bacillus anthracis. This tool aids in assessing exposure risks and improving water quality monitoring.
Area of Science:
- Environmental microbiology
- Molecular diagnostics
- Water quality assessment
Background:
- Traditional water quality monitoring relies on indicator organisms, which may not correlate with pathogen presence.
- Direct detection of multiple microbial pathogens in water is crucial for accurate risk assessment.
Purpose of the Study:
- To develop and validate a DNA microarray for simultaneous detection of key waterborne pathogens and indicator bacteria.
- To provide a tool for comprehensive water safety analysis.
Main Methods:
- Design of a DNA microarray with specific probes for Cryptosporidium parvum, Cryptosporidium hominis, Enterococcus faecium, Bacillus anthracis, and Francisella tularensis.
- Evaluation of the microarray using environmental samples and spiked tap water.
Main Results:
- The microarray successfully detected Enterococcus faecium, Bacillus anthracis, Francisella tularensis, and Cryptosporidium parvum.
- Cryptosporidium hominis detection was achieved using shared probes with Cryptosporidium parvum.
Conclusions:
- A validated microarray assay for specific detection of fecal indicator bacteria and human pathogens in tap water was developed.
- This technology enables simultaneous detection of multiple organisms, enhancing waterborne pathogen exposure risk assessment.
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