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Updated: May 16, 2026

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography
Published on: January 18, 2020
Analysis of glyoxal and related substances by means of high-performance liquid chromatography with refractive index
Zhiyong Zhang1, Dishun Zhao, Baoyun Xu
11Tianjin Key Laboratory of Applied Catalysis Science and Technology and State Key Laboratory for Chemical Engineering (Tianjin University), School of Chemical Engineering, Tianjin University, Tianjin, 300072, China.
Abstract:
A simple and rapid method is described for the analysis of glyoxal and related substances by high-performance liquid chromatography with a refractive index detector. The following chromatographic conditions were adopted: Aminex HPX-87H column, mobile phase consisting of 0.01N H2SO4, flow rate of 0.8 mL/min and temperature of 65°C. The application of the analytical technique developed in this study demonstrated that the aqueous reaction mixture produced by the oxidation of acetaldehyde with HNO3 was composed of glyoxal, acetaldehyde, acetic acid, formic acid, glyoxylic acid, oxalic acid, butanedione and glycolic acid. The method was validated by evaluating analytical parameters such as linearity, limits of detection and quantification, precision, recovery and robustness. The proposed methodology was successfully applied to the production of glyoxal.
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