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Related Experiment Video

Updated: May 16, 2026

Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics
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Published on: September 14, 2015

Predicting stem cell fate changes by differential cell cycle progression patterns.

Marta Roccio1, Daniel Schmitter, Marlen Knobloch

  • 1Laboratory of Stem Cell Bioengineering, Institute of Bioengineering and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

Development (Cambridge, England)
|November 30, 2012
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel single-cell tracking method to analyze cell cycle progression in live stem cells. This technique reveals distinct cell cycle dynamics linked to stem cell self-renewal, differentiation, and reprogramming, enabling precise fate manipulation.

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Area of Science:

  • Stem cell biology
  • Cell cycle regulation
  • Developmental biology

Background:

  • The coordination between cell cycle progression and cell fate determination in stem cells remains poorly understood.
  • Existing methods limit systematic investigation of this relationship in live stem cells.
  • Cell cycle kinetics may offer early predictive markers for stem cell fate changes.

Purpose of the Study:

  • To develop a method for real-time, single-cell analysis of cell cycle phases in stem cells.
  • To identify specific cell cycle signatures associated with stem cell self-renewal, differentiation, and reprogramming.
  • To utilize these signatures for predicting and manipulating stem cell fates.

Main Methods:

  • Developed a single-cell tracking approach using fluorescent ubiquitylation-based cell-cycle indicator (FUCCI) probes.
  • Automated detection and analysis of cell cycle phases (G1, S/G2-M) in live stem cells.
  • Applied fluorescence-activated cell sorting based on FUCCI probe signals to isolate specific cell populations.

Main Results:

  • Identified distinct changes in G1 (red) and S/G2-M (green) phase durations and fluorescence intensities correlating with neural stem/progenitor cell (NSC) and embryonic stem cell (ESC) fates.
  • Demonstrated successful purification of stem cells from heterogeneous populations by sorting cells with lower red fluorescence (longer G1) during NSC differentiation.
  • Showed enhanced reprogramming efficiency by pre-selecting green-fluorescent ESCs (short G1) during NSC to induced pluripotent stem cell conversion.

Conclusions:

  • Established a link between cell cycle kinetics and stem cell fate decisions.
  • The developed FUCCI-based tracking and sorting method allows for prediction and manipulation of cell fate.
  • This approach holds potential for diverse mammalian cell systems to study and control cell fate choices.