Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MonSter++: Unified Stereo Matching, Multi-View Stereo, and Real-Time Stereo With Monodepth Priors.

IEEE transactions on pattern analysis and machine intelligence·2026
Same author

ZNF662 inhibits oncogenesis through NUPR1/p53 signaling pathway in epithelial ovarian cancer and is regulated by hsa-miR-429.

Cellular & molecular biology letters·2026
Same author

Minimally invasive versus open interval debulking surgery for advanced epithelial ovarian cancer: a BMI-stratified analysis.

Surgical endoscopy·2026
Same author

Retraction notice to"The synergistic administration of sanguinarine and curcumin ameliorates indomethacin-induced small intestinal injury in rats through the modulation of Nrf2 and NF-κB signaling pathways" [Biochem. Biophys. Res. Commun. 781 (2025) 152551].

Biochemical and biophysical research communications·2026
Same author

Hypoxia-driven phase separation of the PABP1/eIF4B complex forms stress granules and activates ChaC2 translation to promote polyunsaturated lipids-supported peritoneal metastasis in gastric cancer.

Cancer letters·2026
Same author

Global cancer statistics for children: Two decades of change and projections to 2050.

CA: a cancer journal for clinicians·2026

Related Experiment Video

Updated: May 16, 2026

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Phase retrieval with random phase illumination.

Albert Fannjiang1, Wenjing Liao

  • 1Department of Mathematics, University of California, Davis, California 95616, USA. fannjiang@math.ucdavis.edu

Journal of the Optical Society of America. A, Optics, Image Science, and Vision
|December 4, 2012
PubMed
Summary

Standard phasing algorithms perform well with random phase illumination (RPI). High-resolution RPI ensures a unique solution, leading to rapid convergence and excellent approximations even with noise.

More Related Videos

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Related Experiment Videos

Last Updated: May 16, 2026

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Area of Science:

  • Computational imaging
  • Image reconstruction algorithms

Background:

  • Phasing algorithms are crucial for reconstructing images from diffraction data.
  • Random Phase Illumination (RPI) is a technique used to improve phasing.
  • Understanding RPI's performance with standard algorithms is essential for accurate image reconstruction.

Purpose of the Study:

  • To numerically investigate the performance of standard phasing algorithms using Random Phase Illumination (RPI).
  • To determine the conditions under which RPI ensures unique phasing solutions and rapid convergence.
  • To evaluate the robustness of RPI-based phasing against various noise types.

Main Methods:

  • Detailed numerical simulations were conducted.
  • Standard phasing algorithms were tested with different resolutions of RPI.
  • The oversampling ratio (σ) was varied (σ=4, σ=2, σ=1) to assess its impact.
  • Performance was analyzed in the presence of additive and multiplicative noise.

Main Results:

  • High-resolution RPI with σ=4 guarantees a unique phasing solution (up to a global phase).
  • Standard algorithms rapidly converge to the correct solution without stagnation under these conditions.
  • Excellent approximations were achieved quickly, even with low-resolution RPI and noise.
  • RPI with σ=2 is sufficient for complex-valued images (sector condition), and σ=1 for non-negative images.

Conclusions:

  • RPI significantly enhances the performance and reliability of standard phasing algorithms.
  • The choice of oversampling ratio is critical and depends on image properties (complex-valued vs. non-negative).
  • The error-reduction algorithm with RPI demonstrates convergence to the true solution under specific conditions, validating its effectiveness.