Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-time dynamic single-molecule protein sequencing on an integrated semiconductor device.

Science (New York, N.Y.)·2022
Same author

INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex.

Nature communications·2022
Same author

A Scalable CMOS Molecular Electronics Chip for Single-Molecule Biosensing.

IEEE transactions on biomedical circuits and systems·2022
Same author

Molecular electronics sensors on a scalable semiconductor chip: A platform for single-molecule measurement of binding kinetics and enzyme activity.

Proceedings of the National Academy of Sciences of the United States of America·2022
Same author

Author Correction: Mutations in PYCR1 cause cutis laxa with progeroid features.

Nature genetics·2022
Same author

Ultrasound-on-chip platform for medical imaging, analysis, and collective intelligence.

Proceedings of the National Academy of Sciences of the United States of America·2021
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
Same journal

CMOS Electrokinetic Systems and Fabrication Approaches for On-CMOS 3D Electrodes.

Electrophoresis·2026
See all related articles

Related Experiment Video

Updated: May 16, 2026

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
09:30

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Published on: August 17, 2022

Progress in ion torrent semiconductor chip based sequencing.

Barry Merriman1, , Jonathan M Rothberg

  • 1Ion Torrent, A Division of Life Technologies, Inc., Guilford, CT 06437, USA.

Electrophoresis
|December 5, 2012
PubMed
Summary
This summary is machine-generated.

Semiconductor chip-based DNA sequencing offers a scalable, economical approach for widespread diagnostic use. This technology leverages established semiconductor manufacturing, enhancing throughput, read length, and accuracy for next-generation sequencing.

More Related Videos

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform
06:21

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform

Published on: May 10, 2024

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
09:03

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

Related Experiment Videos

Last Updated: May 16, 2026

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform
09:30

Pre-Implantation Genetic Testing for Aneuploidy on a Semiconductor Based Next-Generation Sequencing Platform

Published on: August 17, 2022

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform
06:21

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform

Published on: May 10, 2024

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
09:03

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

Area of Science:

  • Biotechnology
  • Semiconductor Manufacturing
  • Genomics

Background:

  • Next-generation sequencing (NGS) requires cost-effective, high-volume production for healthcare diagnostics.
  • Complementary metal-oxide semiconductor (CMOS) chip fabrication offers a mature, scalable manufacturing base.
  • Integrating sequencing technology with semiconductor manufacturing is key to achieving widespread adoption.

Purpose of the Study:

  • To provide an overview of semiconductor chip-based DNA sequencing technology.
  • To summarize advancements in this sequencing approach since its commercialization.
  • To detail improvements in chip performance, platform enhancements, and community engagement.

Main Methods:

  • Overview of Ion Torrent's semiconductor chip-based sequencing platform.
  • Analysis of progress in chip scaling, sequencing throughput, read length, and accuracy.
  • Summary of platform enhancements including sample preparation and data processing.

Main Results:

  • Commercialization of a semiconductor chip-based sequencing platform by Ion Torrent.
  • Demonstrated progress in chip scaling, leading to increased sequencing throughput.
  • Improvements in read length and accuracy of the sequencing technology.
  • Enhancements in the associated platform, including sample preparation and data processing.

Conclusions:

  • Semiconductor chip-based sequencing is a viable technology for mass production.
  • Continued advancements are improving the performance and accessibility of NGS diagnostics.
  • Open source and crowdsourcing initiatives are fostering community engagement and further development.