Selective precipitation of large RNAs
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Updated: May 16, 2026

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
Published on: August 18, 2018
Abstract:
For reasons that are somewhat mysterious, "large" RNAs ≥100 nucleotides long can be selectively separated from small RNAs <100 nucleotides long by precipitation with either LiCl or PEG/NaCl. These methods are very straightforward, and are valuable for enriching for very small RNAs (i.e., miRNAs) in unprecipitated material while retaining all other RNAs in the precipitate.
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