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HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
Published on: November 11, 2022
[Study on the HPLC-DAD fingerprint chromatograms of Radix Rubiae from different areas in China]
Xiao Yuan1, Jun-fei Gao, Ping Yuan
1Wuhan Botanic Garden, Chinese Academy of Sciences, Wuhan 430074, China. yuanxiaomail@yahoo.com.cn
Objective:
To establish the HPLC-DAD fingerprint of Radix Rubiae and provide a quality control method for it.
Methods:
The method HPLC-DAD was performed on a YMC C,18 (250 mm x 4.6 mm, 5 microm) column with gradient elution of acetonitrile-0.2% phosphoric acid solution as mobile phase, flow rate at 1.0 mL/min, detection wavelength at 280 mm, and the column temperature at 25 degrees C, and the analysis time was 60 min.
Results:
The average similarity of the fnigerprint chromatograms was over 0.94 in 6 batches samples of different areas in China.
Conclusion:
This method can be applied for the determination of Radix Rubiae from different areas.
