Rapid and sensitive detection of Acinetobacter baumannii using loop-mediated isothermal amplification
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Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025
Po-Chi Soo1, Chun-Chieh Tseng, Siao-Ru Ling
1Department of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien, Taiwan.
Journal of Microbiological Methods
|December 11, 2012
Abstract:
Here we report the design and evaluation of a loop-mediated isothermal amplification (LAMP) assay for detecting Acinetobacter baumannii DNA based on the 16S-23S rRNA intergenic spacer (ITS) sequence. The results showed that target DNA was amplified and visualized within 30min and with a detection limit 100-fold greater than PCR.

