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High-throughput carotenoid profiling using multivariate curve resolution.

Ron Wehrens1, Elisabete Carvalho, Domenico Masuero

  • 1Centre for Research and Innovation, Fondazione Edmund Mach, Via E. Mach 1, 38010 San Michele all'Adige, (TN), Italy. ron.wehrens@fmach.it

Analytical and Bioanalytical Chemistry
|December 11, 2012
PubMed
Summary
This summary is machine-generated.

Automated analysis of high-performance liquid chromatography with diode array detection (HPLC-DAD) data using multivariate curve resolution reveals compound stability. Triethylamine addition enhances the stability of carotenoids, chlorophyll, and tocopherols in grape extracts for up to 30 days.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Chromatography

Background:

  • High-throughput liquid chromatography with diode array detection (HPLC-DAD) generates complex datasets.
  • Analyzing UV-Vis active compounds in mixtures requires advanced data processing techniques.
  • Understanding the stability of natural compounds is crucial for various applications.

Purpose of the Study:

  • To present an automated data analysis method for HPLC-DAD data using multivariate curve resolution (MCR).
  • To evaluate the stability of isoprenoids (carotenoids, chlorophyll, tocopherols) in grape extracts.
  • To demonstrate the effectiveness of triethylamine in preserving compound stability.

Main Methods:

  • Application of multivariate curve resolution (MCR) for automated analysis of HPLC-DAD data.
  • Characterization of spectra and elution profiles for UV-Vis active compounds.
  • Comparative study of grape extracts under different experimental conditions.

Main Results:

  • MCR successfully resolved spectra and elution profiles of UV-Vis active compounds.
  • Quantitative analysis showed significantly enhanced stability of carotenoids, chlorophyll, and tocopherols.
  • Compounds remained stable for at least 30 days in the presence of triethylamine.

Conclusions:

  • Multivariate curve resolution offers an efficient, noninteractive method for analyzing complex HPLC-DAD data.
  • Triethylamine is beneficial for preserving the stability of key isoprenoids in grape extracts.
  • The developed method has potential for routine stability assessment of natural products.