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Updated: May 16, 2026
06:49
Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates
Published on: May 8, 2020
A kinetic method for expeditious detection of pyrophosphate anions at nanomolar concentrations based on a nucleic
Xin Su1, Chen Zhang, Xianjin Xiao
1Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, China.
Chemical Communications (Cambridge, England)
|December 12, 2012
Abstract:
A kinetic method has been developed for highly sensitive and selective detection of pyrophosphate anions (PPi) by using a fluorophore labelled single-stranded-DNA-Al(III) complex system. The facile and spectrum-tunable fluorescent sensor enables rapid detection of PPi in urine and cell lysate solutions without the need for complex pre-cleanup procedures.
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