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Purification of C8 and C9 from rat serum
J Jones1, I Laffafian, B P Morgan
1Department of Medical Biochemistry, University of Wales College of Medicine, Cardiff, UK.
Complement and Inflammation
|January 1, 1990
Abstract:
A procedure based on modifications of published methods for human proteins for the isolation of rat C8 and C9 from one batch of serum is described. The procedure allows the rapid, large-scale isolation of pure and haemolytically active proteins. Rat C9 had a slightly higher molecular weight than human C9 on SDS-PAGE and similar isoelectric point. Rat C8 differed from human C8 in the molecular weight of the gamma chain (23,000 and 21,000 kD respectively), and on isoelectric focusing (pI rat C8: 6.5-6.9; pI human C8: 7.4-7.9).