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Related Concept Videos

DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the lytic replication...
Viral Replication: Lytic Cycle01:20

Viral Replication: Lytic Cycle

Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
Viral Replication: Lysogenic Cycle01:16

Viral Replication: Lysogenic Cycle

The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects its...

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Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System
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Published on: August 17, 2017

Functionalizing bacterial cell surfaces with a phage protein.

Yi-Chun Yeh1, Jana Müller, Changhao Bi

  • 1Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. yichuny@ntnu.edu.tw

Chemical Communications (Cambridge, England)
|December 19, 2012
PubMed
Summary
This summary is machine-generated.

Researchers used a microbe-phage interaction to attach nanoparticles to the surface of bacteria like Escherichia coli. This method offers a new way to engineer bacterial cell surface functions for various applications.

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Area of Science:

  • Microbiology
  • Nanotechnology
  • Biotechnology

Background:

  • Bacterial cell surface functionalization can impart novel properties.
  • Chemical modification is a common approach for altering cell surfaces.
  • Developing new methods for precise surface engineering is crucial.

Purpose of the Study:

  • To demonstrate the use of a bacteriophage-receptor complex for functionalizing bacterial surfaces.
  • To explore the attachment of cadmium selenide/zinc sulfide (CdSe/ZnS) nanoparticles to bacteria.
  • To investigate microbe-phage interactions as a tool for creating new cellular functions.

Main Methods:

  • Utilized a bacteriophage-receptor complex for targeted binding.
  • Applied CdSe/ZnS nanoparticles for surface functionalization.
  • Tested the method on Gram-negative proteobacteria, specifically Escherichia coli and Ralstonia eutropha.

Main Results:

  • Successfully functionalized the surfaces of Escherichia coli and Ralstonia eutropha.
  • Demonstrated the attachment of CdSe/ZnS nanoparticles via the microbe-phage complex.
  • Confirmed the viability of using this interaction for surface engineering.

Conclusions:

  • Microbe-phage interactions provide a novel strategy for bacterial cell surface functionalization.
  • This approach enables the introduction of nanoparticles onto living bacterial cells.
  • Highlights potential for engineering new functionalities on microbial surfaces.