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Complete genome sequence of the NVSL BoHV-1.1 Cooper reference strain
Jean M d'Offay1, Robert W Fulton, R Eberle
1Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, 250 McElroy Hall, Stillwater, OK 74078, USA. jean.doffay@okstate.edu
Archives of Virology
|December 21, 2012
Summary
Researchers sequenced the bovine herpesvirus 1 (BoHV-1) genome, providing a non-chimeric reference strain. This accurate genome sequence is crucial for future molecular genetic studies of BoHV-1.
Area of Science:
- Virology
- Genomics
- Molecular Biology
Background:
- The existing complete genome sequence for bovine herpesvirus 1 (BoHV-1) is a composite, derived from multiple strains.
- This chimeric sequence presents limitations for precise molecular genetic research on BoHV-1.
Purpose of the Study:
- To determine and report the complete genome sequence of the BoHV-1.1 NVSL reference strain Cooper.
- To provide a non-chimeric, accurate genome sequence for BoHV-1 research.
Main Methods:
- Whole-genome sequencing of the BoHV-1.1 NVSL reference strain Cooper.
- Bioinformatic analysis and comparison with the existing chimeric BoHV-1 genome sequence.
Main Results:
- The complete genome sequence of the BoHV-1.1 NVSL reference strain Cooper was successfully determined.
- The Cooper strain genome exhibits nucleotide substitutions, deletions, and insertions compared to the chimeric sequence, with many impacting coding regions.
Conclusions:
- The reported genome sequence of BoHV-1.1 strain Cooper represents a significant advancement over the previous chimeric sequence.
- This accurate reference genome is essential for advancing molecular genetic studies and understanding BoHV-1 biology.
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