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Updated: May 15, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array
Published on: November 15, 2017
Novel stem-loop probe DNA arrays: detection of specific acetotrophic 16S ribosomal RNA signatures
Jonas Boateng1, Robert Zahorchak, Joel Peek
1Department of Chemical Engineering, University of Alabama in Huntsville, Huntsville, AL 35899, USA.
Abstract:
In a recent study, we showed how novel stem-loop DNA probes on dendron-modified aldehyde substrates could be used to detect synthetic nucleic acid targets without amplification. In this article, we demonstrate the application of stem-loop DNA probes as arrays for the detection of specific families and genera of methane-producing bacteria from sludge samples harvested from an anaerobic digester using 16S ribosomal RNA (rRNA) signatures. Specific 16S rRNA could be detected in samples that had 0.2ng/μl total sludge RNA without any target amplification.
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