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Comparison of methods for detecting soluble fibrin in plasma. An in vitro study
S Halvorsen1, O H Skjønsberg, R Ruyter
1Haematological Research Laboratory, Ullevål Hospital, University of Oslo, Norway.
Abstract:
The ability of the COA-SET Fibrin monomer (COA-SET FM) test to detect soluble fibrin was evaluated by comparing the results of the COA-SET FM test with fibrinopeptide A (FPA) determinations following thrombin incubation of plasma or whole blood. In addition, two semiquantitative tests (erythrocytes-agglutination test (FM-test) and ethanol gelation test (EGT] were included in the study. Under the experimental conditions used, the COA-SET FM test proved less sensitive than the FPA-assay. There was a strong correlation between the results obtained by the two tests (r = 0.86, p = 0.0001). When solely regarding low levels of soluble fibrin, however, the correlation was weaker (r = 0.59, p = 0.0003). The FM-test was less sensitive than the COA-SET FM test, but more sensitive than EGT at normal and low fibrinogen concentrations. At high fibrinogen concentrations, however, EGT proved more sensitive than the FM-test. Knowing that 1-2 moles of FPA are released per mole of fibrin monomers formed, a discrepancy was observed between the FPA concentrations and the fibrin monomer concentrations as determined by the COA-SET FM test, the FPA levels being 2-25 times higher than the fibrin monomer levels. The discrepancy was greatest at incipient fibrinogen-fibrin transformation and at high plasma fibrinogen levels. This may suggest that fibrinogen in some way interfered with the stimulating effect of fibrin on the t-PA catalyzed activation of plasminogen, the principle upon which the COA-SET FM test is based.(ABSTRACT TRUNCATED AT 250 WORDS)
Insights
The COA-SET Fibrin monomer (FM) test detects soluble fibrin but is less sensitive than fibrinopeptide A (FPA) assays. Discrepancies suggest fibrinogen may interfere with the COA-SET FM test
Area of Science:
- Coagulation and Hemostasis Research
- Biochemical Assays and Diagnostics
- Thrombosis and Fibrinolysis Studies
Background:
- Soluble fibrin is a marker in thrombotic disorders.
- Accurate detection of soluble fibrin is crucial for clinical diagnostics.
- Existing assays for soluble fibrin have varying sensitivities and specificities.
Purpose of the Study:
- To evaluate the diagnostic performance of the COA-SET Fibrin monomer (FM) test for soluble fibrin detection.
- To compare the COA-SET FM test with fibrinopeptide A (FPA) assays and other semiquantitative tests.
- To investigate potential interferences affecting the COA-SET FM test.
Main Methods:
- Plasma and whole blood samples were incubated with thrombin.
- Soluble fibrin was measured using the COA-SET FM test.
- Fibrinopeptide A (FPA) levels were determined.
- Two semiquantitative tests, the erythrocytes-agglutination test (FM-test) and ethanol gelation test (EGT), were also employed.
Main Results:
- The COA-SET FM test showed lower sensitivity compared to the FPA assay.
- A strong correlation was observed between COA-SET FM and FPA results (r=0.86), but weaker at low soluble fibrin levels (r=0.59).
- FPA levels were 2-25 times higher than fibrin monomer levels, indicating a discrepancy potentially due to fibrinogen interference.
Conclusions:
- The COA-SET FM test is less sensitive than FPA assays for soluble fibrin detection.
- Fibrinogen may interfere with the COA-SET FM test's principle, affecting its accuracy.
- Further research is needed to optimize soluble fibrin detection methods.