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Updated: May 15, 2026

In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse
Published on: October 25, 2024
[Expression of Th17 related cell factors and its significance in CRSsNP]
Baofeng Wang1, Bennong Li, Jing Cao
1Department of Otorhinolaryngology, Puai Hospital, Wuhan,430034, China.
Objective:
To detect the expression of Th17 nuclear factor RORC and cytokines IL-17A and IL-22 and neutrophil marker MPO and their correlations with CRSsNP.
Method:
RT-PCR was used to detect mRNA expression of RORC, IL-17A and IL-22. Immunohistochemistry was used to assess the IL-17A positive cells in CRSsNP and control. ELISA was used to detect the expression of MPO.
Result:
CRSsNP had higher mRNA expression of RORC, IL-17A and IL-22 and increased protein expression of MPO. The mRNA expression of RORC, IL-17A and IL-22 was positively correlated with each other but none of them was correlated with the expression of MPO in CRSsNP and control.
Conclusion:
Both Th17 and neutrophils contribute to the pathogenesis of CRSsNP, however, the neutrophil infiltration may not be recruited by Th17 cytokines.
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