Related Experiment Video
Updated: May 15, 2026

09:06
Cryo-EM and Single-Particle Analysis with Scipion
Published on: May 29, 2021
STEM_CELL: a software tool for electron microscopy: part 2--analysis of crystalline materials
Vincenzo Grillo1, Francesca Rossi
1Centro S3, CNR-Istituto di Nanoscienze, Via Campi 213A, I-41125 Modena, Italy. vincenzo.grillo@unimore.it
Ultramicroscopy
|January 1, 2013
Summary
A new graphical software, STEM_CELL, offers advanced analysis for HRTEM and STEM-HAADF images. It introduces novel methods for atomic-scale quantitative analysis and extended geometric phase analysis, improving materials science research.
Area of Science:
- Materials Science
- Electron Microscopy
- Image Analysis
Background:
- High-resolution transmission electron microscopy (HRTEM) and scanning transmission electron microscopy-high-angle annular dark-field (STEM-HAADF) are crucial for materials characterization.
- Existing software often lacks integrated analysis and simulation capabilities for advanced imaging techniques.
- Developing novel methodologies for quantitative and large-scale analysis is essential for deeper insights.
Purpose of the Study:
- Introduce STEM_CELL, a new graphical software for HRTEM and STEM-HAADF image analysis.
- Present novel analysis methodologies by integrating diverse algorithms and simulations.
- Highlight key methodological advancements in atomic-scale and large-region analysis.
Main Methods:
- Development of a user-friendly graphical software interface (STEM_CELL).
- Integration of various analysis algorithms and simulation tools.
- Implementation of improved image processing techniques including filtering, normalization, and background subtraction.
Main Results:
- A procedure for atomic-scale quantitative analysis of HAADF images has been established.
- Geometric phase analysis has been extended to analyze large regions (up to 1μm) using undersampled images.
- Novel analysis methodologies combining different algorithms and simulations are presented.
Conclusions:
- STEM_CELL provides a powerful and versatile platform for advanced electron microscopy image analysis.
- The software enables new possibilities for quantitative and large-scale materials characterization.
- The presented methodologies push the boundaries of current electron microscopy analysis techniques.

