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Updated: May 15, 2026

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
Published on: November 28, 2017
Chemical proteomics: ligation and cleavage of protein modifications
Georg C Rudolf1, Wolfgang Heydenreuter, Stephan A Sieber
1Chair of Organic Chemistry II, Department of Chemistry, Technical University of Munich, Garching 85747, Germany.
Abstract:
Bioorthogonal ligation and cleavage methods are of major importance in the field of chemical biology. Recently, there has been significant progress in the improvement of classic ligation procedures as well as in the establishment of new ligation methodologies. Furthermore, the design of cleavable linkers for protein enrichment has lately received much attention. These techniques equip researchers with a wealth of tools suitable for proteomic applications. In order to ease navigation through these diverse systems, we here provide a comprehensive overview of methods that are useful for chemical proteomics.
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