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Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
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In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse

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Functional human Th17 clones with WT1-specific helper activity.

Sho Tachino1, Fumihiro Fujiki, Yoshihiro Oka

  • 1Department of Respiratory Medicine, Allergy and Rheumatic Diseases, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, Japan. stachino@imed3.med.osaka-u.ac.jp

Cancer Immunology, Immunotherapy : CII
|January 1, 2013
PubMed
Summary
This summary is machine-generated.

Researchers established functional T helper 17 (Th17) clones specific to the Wilms' tumor 1 (WT1) antigen. These antigen-specific Th17 clones can help conventional CD4(+) T cells proliferate, offering a new tool for studying Th17 roles in tumor immunity.

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Area of Science:

  • Immunology
  • Oncology
  • Cellular Biology

Background:

  • T helper 17 (Th17) cells are crucial in autoimmune and inflammatory diseases.
  • The role of Th17 cells in anti-tumor immunity is increasingly recognized but not fully understood, particularly in humans.
  • Wilms' tumor 1 (WT1) is a tumor-associated antigen implicated in various cancers.

Purpose of the Study:

  • To establish and characterize WT1 antigen-specific Th17 clones from human peripheral blood mononuclear cells.
  • To investigate the functional capabilities of these antigen-specific Th17 clones, specifically their helper activity.
  • To provide a novel tool for dissecting the precise roles of human Th17 cells in the context of tumor immunity.

Main Methods:

  • Generation of WT1-specific Th17 clones using a WT1-derived helper peptide (WT1332) under Th17-polarizing conditions.
  • Stimulation of peripheral blood mononuclear cells (PBMCs) with the WT1332 peptide.
  • Assessment of the helper function of established Th17 clones on conventional CD4(+) T cell proliferation.

Main Results:

  • Successful establishment of WT1-specific Th17 clones.
  • Demonstration that the established WT1-specific Th17 clone possesses antigen-specific helper activity.
  • The Th17 clone promoted the proliferation of conventional CD4(+) T cells in a manner dependent on antigenic stimulation.

Conclusions:

  • This study reports the first successful establishment of functional human Th17 clones with both WT1 antigen specificity and antigen-specific helper activity.
  • The developed method for generating antigen-specific Th17 clones provides a valuable resource for future research.
  • These findings pave the way for deeper investigation into the complex roles of Th17 cells in human tumor immunity.