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Arylalkylamine (serotonin) N-acetyltransferase assay using high-performance liquid chromatography with fluorescence
K B Thomas1, J Zawilska, P M Iuvone
1Emory University School of Medicine, Department of Pharmacology, Atlanta, Georgia 30322.
Analytical Biochemistry
|February 1, 1990
Abstract:
A sensitive, rapid, and economical method has been developed for determination of serotonin N-acetyltransferase activity from a variety of enzyme sources. The assay is based upon separation and detection of N-acetyltryptamine formed from tryptamine and acetyl coenzyme A, by means of high-performance liquid chromatography with either electrochemical or fluorometric detection. The limit of sensitivity with both detection methods is less than 20 pmol of N-acetyltryptamine formed per sample. A method for synthesis of N-acetyltryptamine, used as an external standard in the assay, is described.